解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-depe...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00453-2
更新日期:2003-12-01 00:00:00
abstract::The functional significance of mono-, di-, and trimethylation of lysine residues within histone proteins remains unclear. Antibodies developed to selectively recognize each of these methylated states at histone H3 lysine 9 (H3 Lys9) demonstrated that mono- and dimethylation localized specifically to silent domains wit...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00479-9
更新日期:2003-12-01 00:00:00
abstract::Integration of the mycobacteriophage Bxb1 genome into its host chromosome is catalyzed by a serine-integrase, a member of the transposon-resolvase family of site-specific recombinases. These enzymes use a concerted mechanism of strand exchange involving double-stranded cleavages with two-base extensions, and covalent ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00444-1
更新日期:2003-11-01 00:00:00
abstract::The bHLH factors HAND1 and HAND2 are required for heart, vascular, neuronal, limb, and extraembryonic development. Unlike most bHLH proteins, HAND factors exhibit promiscuous dimerization properties. We report that phosphorylation/dephosphorylation via PKA, PKC, and a specific heterotrimeric protein phosphatase 2A (PP...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00425-8
更新日期:2003-11-01 00:00:00
abstract::Members of the ISWI family of chromatin remodeling factors exhibit ATP-dependent nucleosome sliding, loading, and spacing activities in vitro. However, it is unclear which of these activities are utilized by ISWI complexes to remodel chromatin in vivo. We therefore sought to identify the mechanisms of chromatin remode...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00436-2
更新日期:2003-11-01 00:00:00
abstract::An exciting study in this issue reveals how binding of the microtubule associated protein TPX2 to the mitotic kinase Aurora A induces a conformational change. This moves the phosphorylated activation domain into a more compact position within the kinase core, providing a better substrate binding platform and hiding th...
journal_title:Molecular cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s1097-2765(03)00405-2
更新日期:2003-10-01 00:00:00
abstract::In order to determine the global effects of oncogenic Ras and Akt signaling pathways on translational efficiencies, we compared the gene expression profiles of total cellular mRNA and mRNA associated with polysomes. We found that the immediate effect of Ras and Akt signaling blockade on transcription was relatively mo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00395-2
更新日期:2003-10-01 00:00:00
abstract::The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. Density due to TFIIF was not concentrated in one area but rather was widely distributed across the surface of the polymerase. The largest subunit of TFIIF interacted...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00387-3
更新日期:2003-10-01 00:00:00
abstract::The interaction between pRB and E2F is critical for control of the cell cycle and apoptosis. Here we report that pRB contains two distinct E2F binding sites. The previously identified E2F binding site on pRB is necessary for stable association with E2Fs on DNA. A second E2F interaction site is located entirely within ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00344-7
更新日期:2003-09-01 00:00:00
abstract::The double Holliday junction (dHJ) is generally regarded to be a key intermediate of meiotic recombination, whose resolution is critical for the formation of crossover recombinants. In fission yeast, the Mus81-Eme1 endonuclease has been implicated in resolving dHJs. Consistent with this role, we show that Mus81-Eme1 i...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00343-5
更新日期:2003-09-01 00:00:00
abstract::Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00278-8
更新日期:2003-08-01 00:00:00
abstract::Activation of retrograde signaling (RS) by mitochondrial dysfunction or by inhibition of TOR kinases in yeast results in nuclear accumulation of the transcription factors, Rtg1p and Rtg3p. This process requires Rtg2p, a novel cytoplasmic protein with an N-terminal ATP binding domain. We show that Rtg2p controls RS by ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00285-5
更新日期:2003-08-01 00:00:00
abstract::In yeast Saccharomyces cerevisiae the G protein betagamma subunits (Ste4/Ste18) have long been known to transmit the signal required for mating. Here we demonstrate that GTPase-deficient mutants of Galpha (Gpa1) directly activate the mating response pathway. We also show that signaling by activated Gpa1 requires direc...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00307-1
更新日期:2003-08-01 00:00:00
abstract::Bcr-Abl is a dysregulated tyrosine kinase whose mechanism of activation is unclear. Here, we demonstrate that, like c-Abl, Bcr-Abl is negatively regulated through its SH3 domain. Kinase activity, transformation, and leukemogenesis by Bcr-Abl are greatly impaired by mutations of the Bcr coiled-coil domain that disrupt ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00274-0
更新日期:2003-07-01 00:00:00
abstract::Actin-related proteins (Arps) and conventional actin are enigmatic components of many chromatin-remodeling enzyme complexes. The yeast INO80 ATP-dependent chromatin-remodeling complex contains stoichiometric amounts of Arp4, Arp5, Arp8, and actin. Here we have revealed functions of Arp5 and Arp8 by analysis of mutants...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00264-8
更新日期:2003-07-01 00:00:00
abstract::Small DNA viruses force quiescent cells to reenter the cell cycle in order to replicate their DNA. We report here that the adenovirus E1A protein creates an S phase environment in quiescent cells by overcoming the nucleosomal repression of E2F-targeted genes. These genes are surrounded by Lys-9-methylated H3 histones,...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00225-9
更新日期:2003-07-01 00:00:00
abstract::Tumor suppressor genes evolved as negative effectors of mitogen and nutrient signaling pathways, such that mutations in these genes can lead to pathological states of growth. Tuberous sclerosis (TSC) is a potentially devastating disease associated with mutations in two tumor suppressor genes, TSC1 and 2, that function...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00220-x
更新日期:2003-06-01 00:00:00
abstract::The orphan nuclear receptors SF-1 and LRH-1 are constitutively active, but it remains uncertain whether their activation is hormone dependent. We report the crystal structure of the LRH-1 ligand binding domain to 2.4 A resolution and find the receptor to be a monomer that adopts an active conformation with a large but...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00236-3
更新日期:2003-06-01 00:00:00
abstract::Pilin assembly into type IV pili is required for virulence by bacterial pathogens that cause diseases such as cholera, pneumonia, gonorrhea, and meningitis. Crystal structures of soluble, N-terminally truncated pilin from Vibrio cholera toxin-coregulated pilus (TCP) and full-length PAK pilin from Pseudomonas aeruginos...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00170-9
更新日期:2003-05-01 00:00:00
abstract::Dosage compensation ensures equal expression of X-linked genes in males and females. In Drosophila, equalization is achieved by hypertranscription of the male X chromosome. This process requires an RNA/protein containing dosage compensation complex (DCC). Here we use RNA interference of individual DCC components to de...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00140-0
更新日期:2003-05-01 00:00:00
abstract::Telomeres protect chromosome ends from fusing to double-stranded breaks (DSBs). Using a quantitative real-time PCR assay, we show that nonhomologous end joining between a telomere and an inducible DSB was undetectable in wild-type cells, but occurred within a few hours of DSB induction in approximately 1/2000 genomes ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00174-6
更新日期:2003-05-01 00:00:00
abstract::MazF and MazE are components of a chromosomal toxin-antitoxin system of Escherichia coli. In this issue of Molecular Cell, Kamada et al. describe the crystal structure of a MazE/MazF heterohexamer and propose that the mechanism of toxin-antidote recognition is common to other homologous chromosomal and plasmid-borne s...
journal_title:Molecular cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s1097-2765(03)00146-1
更新日期:2003-04-01 00:00:00
abstract::Messenger RNAs with premature translation termination codons (PTCs) are degraded by nonsense-mediated mRNA decay (NMD). In mammals, PTCs are discriminated from physiological stop codons by a process thought to involve the splicing-dependent deposition of an exon junction complex (EJC), EJC-mediated recruitment of Upf3...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00142-4
更新日期:2003-04-01 00:00:00
abstract::SPT5 and its binding partner SPT4 function in both positively and negatively regulating transcriptional elongation. The demonstration that SPT5 and RNA polymerase II are targets for phosphorylation by CDK9/cyclin T1 indicates that posttranslational modifications of these factors are important in regulating the elongat...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00101-1
更新日期:2003-04-01 00:00:00
abstract::Replication forks frequently break and must be repaired by recombination. A reconstituted reaction now allows the factors that coordinate conversion from a recombination intermediate back to a replication fork to be defined. The PriA protein plays a key role in this control. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s1097-2765(03)00110-2
更新日期:2003-03-01 00:00:00
abstract::ClpXP is a protease involved in DNA damage repair, stationary-phase gene expression, and ssrA-mediated protein quality control. To date, however, only a handful of ClpXP substrates have been identified. Using a tagged and inactive variant of ClpP, substrates of E. coli ClpXP were trapped in vivo, purified, and identif...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00060-1
更新日期:2003-03-01 00:00:00
abstract::Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifica...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00069-8
更新日期:2003-03-01 00:00:00
abstract::One fundamental function of telomeres is to prevent the ends of chromosomes from being sensed and treated as DNA damage. Here we present evidence for additional roles of telomeres in promoting proper chromosome segregation and DNA repair. We find that the fission yeast telomere protein Taz1p is required for cell cycle...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00041-8
更新日期:2003-02-01 00:00:00
abstract::One of the oldest questions in RNA science is the role of nucleotide modification. Here, the importance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depleting yeast cells of 1-5 snoRNAs that guide a total of six Psi modifications. Translation was impaired substantially wi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00040-6
更新日期:2003-02-01 00:00:00
abstract::In the death receptor induced apoptotic pathway, caspase-8 autocatalytically cleaves itself at specific cleavage sites. To better understand the regulatory mechanisms behind caspase-8 activation, we compared active wild-type caspase-8 (wtC8) and an uncleavable form of procaspase-8 (uncleavable C8). We demonstrate that...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00059-5
更新日期:2003-02-01 00:00:00
abstract::The binding of cytoplasmic proteins, such as talin, to the cytoplasmic domains of integrin adhesion receptors mediates bidirectional signal transduction. Here we report the crystal structure of the principal integrin binding and activating fragment of talin, alone and in complex with fragments of the beta 3 integrin t...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00823-7
更新日期:2003-01-01 00:00:00
abstract::Examination of the binding of FeBABE-conjugated BvgA to the fha promoter of Bordetella pertussis has revealed that three dimers, formed by head-to-head association of monomers, bind one face of the DNA helix from the inverted-heptad primary binding site to the -35 region. The orientation of BvgA monomers within the di...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00007-8
更新日期:2003-01-01 00:00:00
abstract::The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00824-9
更新日期:2003-01-01 00:00:00
abstract::One of the prominent biological phenomena controlled by Wnt signaling is the expansion of cells with predefined fates. In the November 27, 2002, issue of Cell, Rosenfeld and colleagues describe an example of Wnt-controlled proliferation during genesis of the cardiac outflow tract and the pituitary gland. As a downstre...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00801-8
更新日期:2002-12-01 00:00:00
abstract::Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00727-x
更新日期:2002-12-01 00:00:00
abstract::We analyzed the order of recruitment of factors to the HNF-4alpha regulatory regions upon the initial activation of the gene during enterocyte differentiation. An initially independent assembly of regulatory complexes at the proximal promoter and the upstream enhancer regions was followed by the tracking of the entire...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00786-4
更新日期:2002-12-01 00:00:00
abstract::While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combin...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00749-9
更新日期:2002-11-01 00:00:00
abstract::Mutations in Hnf-1alpha are the most common Mendelian cause of diabetes mellitus. To elucidate the molecular function of a mutational hotspot, we cocrystallized human HNF-1alpha 83-279 with a high-affinity promoter and solved the structure of the complex. Two identical protein molecules are bound to the promoter. Each...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00704-9
更新日期:2002-11-01 00:00:00
abstract::APOBEC1 is the catalytic component of an RNA editing complex but shows homology to activation-induced cytidine deaminase (AID), a protein whose function is to potentiate diversification of immunoglobulin gene DNA. Here, we show that APOBEC1 and its homologs APOBEC3C and APOBEC3G exhibit potent DNA mutator activity in ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00742-6
更新日期:2002-11-01 00:00:00
abstract::Bcl-2 proteins are critical regulators of apoptosis following DNA damage. Recent studies have shown that genotoxic stress induces the deamidation of Bcl-X(L), inhibiting its antiapoptotic activities. Remarkably, Rb and p53 are important regulators of this novel modification of Bcl-X(L). ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00693-7
更新日期:2002-10-01 00:00:00